14-3-3 基因 AaGRF1 正向调节猕猴桃的耐寒性
Plant Science ( IF 4.2 ) Pub Date : 2025-01-29 , DOI: 10.1016/j.plantsci.2025.112403
Zhang Zhenzhen 1 , Li Sumei 2 , Sun Shihang 1 , Li Hongli 3 , Zhang Qina 2 , Li Yihang 2 , Li Yukuo 1 , Liu Mingyu 2 , Li Congcong 2 , Sun Leiming 2 , Lin Miaomiao 1 , Qi Xiujuan 1
Affiliation
- National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China; Zhongyuan Research Center, Chinese Academy of Agricultural Sciences, Xinxiang 453500, China.
- National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China.
- Mudanjiang Branch of Heilongjiang Academy of Forestry Sciences, Mudanjiang 157000, China.
低温严重威胁着猕猴桃的生长和发育。研究表明，属于 14-3-3 家族的蛋白质在植物抵抗压力的能力中起着关键的调节作用。然而，基因在猕猴桃耐寒性中的这种特定作用仍不清楚。已经确定 β-淀粉酶基因 AaBAM3.1 对猕猴桃的耐低温表现出正向调控作用。在我们的研究中，我们从 AaBAM3.1 启动子的酵母单杂交 （Y1H） 筛选文库中获得了 Actinidia arguta 14-3-3 基因一般调节因子 1 （AaGRF1）;低温胁迫提高了 AaGRF1 的表达水平。亚细胞定位、Y1H 和双 LUC 测定表明 AaGRF1 蛋白位于细胞核内，具有与 AaBAM3.1 启动子相互作用的能力。此外，我们还研究了 AaGRF1 基因在猕猴桃抗寒性中的作用。当 AaGRF1 在猕猴桃中过表达时，转基因植株表现出更强的耐寒性。与野生型 （WT） 品系相比，这些植物中的抗氧化剂和可溶性糖水平升高。转基因和 WT 系的 RNA-seq 显示 AaGRF1 可能与 'ascorbate-glutathione' 和 'starch and sucrose' 途径中的基因相互作用，从而增强猕猴桃的抗寒性。总之，我们假设 14-3-3 基因 AaGRF1 可能通过积累更多的抗氧化剂和可溶性糖来积极调节猕猴桃的抗寒性。
The 14–3–3 gene AaGRF1 positively regulates cold tolerance in kiwifruit
Low temperatures severely threaten the growth and development of kiwifruit. Research has demonstrated that proteins belonging to the 14–3–3 family play a pivotal regulatory function in the ability of plants to resist stress. However, this specific roles of the genes in kiwifruit cold tolerance remain unclear. It had been identified that β-amylase gene, AaBAM3.1, exhibits a positive regulatory effect on kiwifruit's tolerance to low temperature. In our research, we obtained the Actinidia arguta 14–3–3 gene general regulatory factor 1 (AaGRF1) from yeast one-hybrid (Y1H) screening library of the AaBAM3.1 promoter; the expression level of AaGRF1 was enhanced by low-temperature stress. Subcellular localization, Y1H and dual-LUC assay indicated that the AaGRF1 protein resides within the nucleus and possesses the ability to interact with the AaBAM3.1 promoter. Moreover, we also studied the role of AaGRF1 gene in cold resistance of kiwifruit. When AaGRF1 was overexpressed in kiwifruit, the transgenic plants exhibited enhanced cold tolerance. The level of antioxidants and soluble sugars in these plants were elevated compared to wild-type (WT) lines. RNA-seq of the transgenic and WT lines revealed that AaGRF1 might interact with genes in the ‘ascorbate-glutathione’ and ‘starch and sucrose’ pathways, thereby enhancing the cold resistance of kiwifruit. In summary, we hypothesize that the 14–3–3 gene AaGRF1 may positively modulate the cold resistance in kiwifruit by accumulating more antioxidants and soluble sugars.
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